Spinal sumatriptan inhibits capsaicin-induced canine external carotid vasodilatation via 5-HT1B rather than 5-HT1D receptors

E Munoz-Islas, J Lozano-Cuenca, A Gonzalez-Hernandez, Martha Ramirez Rosas, A Sanchez-Lopez, D Centurion, Antoinette Maassen van den Brink, CM Villalon Herrera

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Migraine is a neurovascular disorder associated with trigeminal activation, vasodilatation and trigeminal release of calcitonin gene-related peptide (CGRP). The antimigraine properties of triptans may be due to: i) vasoconstriction of the carotid arterial bed via 5-HT1B receptors; and ii) inhibition of CGRP release from trigeminal nerves, via 5-HT1B/1D receptors. This study investigated the effects of intrathecally administered sumatriptan (a 5-HT1B/1D receptor agonist) and PNU-142633 (a 5-HT1D receptor agonist) on the canine external carotid vasodilator responses to capsaicin, alpha-CGRP and acetylcholine. For this purpose, 42 mongrel dogs were anaesthetised with sodium pentobarbitone and, subsequently, vagosympathectomized. The animals were prepared to measure arterial blood pressure, heart rate and external carotid blood flow; the thyroid artery was cannulated for infusion of agonists. 1-min intracarotid (i.c.) continuous infusions of capsaicin, alpha-CGRP and acetylcholine produced dose-dependent increases in external carotid blood flow without affecting arterial blood pressure or heart rate. These vasodilator responses remained unaffected after intrathecal (i.t.) administration of physiological saline (0.5 ml) or PNU-142633 (300-1000 mu g); in contrast, i.t. sumatriptan (300-1000 mu g) significantly inhibited the vasodilator responses to capsaicin, but not those to alpha-CGRP or acetylcholine. Furthermore, i.t. administration of SB224289 (a 5-HT1B receptor antagonist), but not of BRL15572 (a 5-HT1D receptor antagonist), abolished the above inhibition by sumatriptan. These results suggest that sumatriptan-induced inhibition of the external carotid vasodilatation to capsaicin involves a central mechanism mainly mediated by 5-HT1B receptors. (C) 2009 Elsevier B.V. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)133-138
Number of pages6
JournalEuropean Journal of Pharmacology
Issue number1-3
Publication statusPublished - 2009

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